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calcein am pl double staining kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology calcein am pl double staining kit
    Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of <t>Calcein-AM</t> (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure
    Calcein Am Pl Double Staining Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 140 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+and+pi+double+staining/Calcein+AM%2FPI+Double+Staining+Kit/pmc13127088-34-0-8
    Average 95 stars, based on 140 article reviews
    calcein am pl double staining kit - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor"

    Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-026-00674-9

    Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of Calcein-AM (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure
    Figure Legend Snippet: Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of Calcein-AM (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure

    Techniques Used: CCK-8 Assay, Control, Fluorescence, Staining

    Related Articles

    Double Staining:

    Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor.
    Article Snippet: .. Calcein AM/Pl double staining kit was obtained from Elabscience (Wuhan, China). .. Mouse IL-1β enzyme-linked immunosorbent assay (ELISA) kit, primary antibodies of NLRP3, ASC, GSDMD, and IL-1β were obtained from ABclonal (Wuhan, China).

    Article Title: Synthesis and Biological Activity Characterization of Vascular Endothelial Growth Factor Using an Optimized Wheat Germ Cell-Free System
    Article Snippet: Primary and secondary antibodies for detection, including the 6 × His-Tag Monoclonal Antibody and HRP-conjugated Goat Anti-Mouse IgG, were sourced from Proteintech Group (Wuhan, China). .. The Calcein AM/PI Double Staining Kit was from Elabscience (Wuhan, China). .. Actin-Tracker Red-Rhodamine, Hoechst 33342, Click-iT EdU-488 Cell Proliferation Assay Kit and recombinant protein PDI were obtained from Beyotime Biotechnology (Shanghai, China).

    Article Title: Ganoderma lucidum polysaccharide-decorated extracellular vesicle enables synergistical antitumor immunotherapy
    Article Snippet: The mouse spleen lymphocyte separation solution, crystal violet staining, and TMR (red) tunel cell apoptosis detection kits were purchased from Solarbio (China). .. Calcein AM/PI double staining kit was purchased from Elabscience (China). ..

    Article Title: In Situ Tantalum Doping of Titanium Dioxide Nanotubes via Electrochemical Method for Enhanced Mechanical and Biological Properties.
    Article Snippet: .. Live/dead cell staining was performed using a Calcein AM/PI Double Staining Kit (Elabscience, Wuhan, China). ..

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. RSC96 and HUVECs (2 × 103 cells/well) were respectively seeded into 96-well culture plates, followed by the addition of supernatants supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) and 1 % penicillin/streptomycin (P/S; PB180120 , Procell, Wuhan, China) after 4 h. Calcein AM/PI double staining kit (E-CK-A354, Elabscience, Wuhan, China) was employed to stain the cells on the first day, with imaging conducted using a fluorescence microscope (CKX53, Olympus, Japan). ..

    Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor
    Article Snippet: .. Calcein AM/Pl double staining kit was obtained from Elabscience (Wuhan, China). .. Mouse IL-1β enzyme-linked immunosorbent assay (ELISA) kit, primary antibodies of NLRP3, ASC, GSDMD, and IL-1β were obtained from ABclonal (Wuhan, China).

    CCK-8 Assay:

    Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
    Article Snippet: .. Cell viability was assessed using the CCK-8 assay (HY-K0301, MedChemExpress, NJ, USA), a scratch wound-healing assay, and Calcein AM/PI dual-fluorescence staining (E-CK-A354, Elabscience, Wuhan, China). ..

    Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling.
    Article Snippet: .. Cell viability was assessed using the CCK-8 assay (HYK0301, MedChemExpress, NJ, USA), a scratch woundhealing assay, and Calcein AM/PI dual-fluorescence staining (E-CK-A354, Elabscience, Wuhan, China). ..

    Wound Healing Assay:

    Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
    Article Snippet: .. Cell viability was assessed using the CCK-8 assay (HY-K0301, MedChemExpress, NJ, USA), a scratch wound-healing assay, and Calcein AM/PI dual-fluorescence staining (E-CK-A354, Elabscience, Wuhan, China). ..

    Staining:

    Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling
    Article Snippet: .. Cell viability was assessed using the CCK-8 assay (HY-K0301, MedChemExpress, NJ, USA), a scratch wound-healing assay, and Calcein AM/PI dual-fluorescence staining (E-CK-A354, Elabscience, Wuhan, China). ..

    Article Title: Optimized New Shengmai Powder suppresses ferroptosis in ischemic cardiomyocytes via cGMP-PKG signalling.
    Article Snippet: .. Cell viability was assessed using the CCK-8 assay (HYK0301, MedChemExpress, NJ, USA), a scratch woundhealing assay, and Calcein AM/PI dual-fluorescence staining (E-CK-A354, Elabscience, Wuhan, China). ..

    Article Title: In Situ Tantalum Doping of Titanium Dioxide Nanotubes via Electrochemical Method for Enhanced Mechanical and Biological Properties.
    Article Snippet: .. Live/dead cell staining was performed using a Calcein AM/PI Double Staining Kit (Elabscience, Wuhan, China). ..

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. RSC96 and HUVECs (2 × 103 cells/well) were respectively seeded into 96-well culture plates, followed by the addition of supernatants supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) and 1 % penicillin/streptomycin (P/S; PB180120 , Procell, Wuhan, China) after 4 h. Calcein AM/PI double staining kit (E-CK-A354, Elabscience, Wuhan, China) was employed to stain the cells on the first day, with imaging conducted using a fluorescence microscope (CKX53, Olympus, Japan). ..

    Imaging:

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. RSC96 and HUVECs (2 × 103 cells/well) were respectively seeded into 96-well culture plates, followed by the addition of supernatants supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) and 1 % penicillin/streptomycin (P/S; PB180120 , Procell, Wuhan, China) after 4 h. Calcein AM/PI double staining kit (E-CK-A354, Elabscience, Wuhan, China) was employed to stain the cells on the first day, with imaging conducted using a fluorescence microscope (CKX53, Olympus, Japan). ..

    Fluorescence:

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. RSC96 and HUVECs (2 × 103 cells/well) were respectively seeded into 96-well culture plates, followed by the addition of supernatants supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) and 1 % penicillin/streptomycin (P/S; PB180120 , Procell, Wuhan, China) after 4 h. Calcein AM/PI double staining kit (E-CK-A354, Elabscience, Wuhan, China) was employed to stain the cells on the first day, with imaging conducted using a fluorescence microscope (CKX53, Olympus, Japan). ..

    Microscopy:

    Article Title: Composite hydrogel-microsphere delivery system promotes early nerve-mediated bone regeneration and late-stage mechanotransduction-driven bone remodeling via sequential release of NGF and Yoda1
    Article Snippet: .. RSC96 and HUVECs (2 × 103 cells/well) were respectively seeded into 96-well culture plates, followed by the addition of supernatants supplemented with 10 % fetal bovine serum (FBS; Gibco, USA) and 1 % penicillin/streptomycin (P/S; PB180120 , Procell, Wuhan, China) after 4 h. Calcein AM/PI double staining kit (E-CK-A354, Elabscience, Wuhan, China) was employed to stain the cells on the first day, with imaging conducted using a fluorescence microscope (CKX53, Olympus, Japan). ..



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    Elabscience Biotechnology calcein am pl double staining kit
    Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of <t>Calcein-AM</t> (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure
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    Image Search Results


    Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of Calcein-AM (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure

    Journal: Particle and Fibre Toxicology

    Article Title: Aged automobile cabin air filters as secondary pollution sources: mechanistic insights into NLRP3 inflammasome activation and discovery of a natural inhibitor

    doi: 10.1186/s12989-026-00674-9

    Figure Lengend Snippet: Dose-dependent cytotoxic effects in ACAFP-exposed macrophages. Cell viability of MH-S cells exposed to different concentrations of A inside ACAFP and B outside ACAFP for 12 and 24 h determined by CCK-8 assay. Data are presented as percentage of control. C Representative fluorescence images of Calcein-AM (green, live cells) and PI (red, dead cells) co-staining after 24 h ACAFP exposure

    Article Snippet: Calcein AM/Pl double staining kit was obtained from Elabscience (Wuhan, China).

    Techniques: CCK-8 Assay, Control, Fluorescence, Staining